Sprecher
Beschreibung
Dynamic DNA nanostructures (DONs) can be used as triggerable switches in novel chemical and biotechnological applications, including electrochemical sensing. One such example is a so-called Zipper DON [1] that can adopt either a closed or open conformation based on the formation of pH-sensitive DNA triplexes. This property makes the Zippers excellent sensor components for pH-dependent sensing devices. Nanopipette as an electrochemical biosensor is an emerging class of low-cost, rapid sensing devices that measure changes to the surface charge of its internal walls. The surface charge creates an electrical double layer overlap at the tip, resulting in a non-ohmic electrical response known as ion current rectification. Here, in combination with the Zippers, the device allows for a highly sensitive and tunable pH-relevant biosensor as the DON modulates the ion current rectification based on its pH-dependent conformation.[2]
[1] P. Williamson, H. Ijäs, B. Shen, D. K. Corrigan, and V. Linko, “Probing the Conformational States of a pH-Sensitive DNA Origami Zipper via Label-Free Electrochemical Methods,” Langmuir, vol. 37, no. 25, pp. 7801–7809, Jun. 2021, doi: 10.1021/acs.langmuir.1c01110.
[2] R. Davis, O. Reinsalu, V. Linko and R. Johnson, article in preparation, 2025
