Sprecher
Beschreibung
DNA origami nanotechnology has seen widespread use in research, yet its translation into commercial products remains limited.
In the context of enabling technologies for drug discovery, we developed and commercialized DNA origami nanolevers as functional biosensing elements for analyzing binding-induced conformational changes in proteins.
These proteins are tethered to a chip surface via DNA origami levers. When subjected to alternating electric fields, the levers oscillate, moving the attached proteins through solution. The nanolever dynamics are influenced by the hydrodynamic friction of the tethered proteins, enabling detection of changes in their 3D conformation through measurements of switching speed. This assay is well-suited for high-throughput screening of drug candidates based on their ability to induce conformational changes in target proteins.
We describe the rationale behind using DNA origami to create distinct advantages for end users, and discuss practical considerations including manufacturability, product quality, and the establishment of a global supply chain for ready-to-use DNA origami kits. Application examples illustrate the analysis of protein conformational changes in workflows compatible with high-throughput screening.
