7th Functional DNA Nanotechnology Workshop

Optimizing Large-Scale DNA Origami Lattices on Silicon

Nicht eingeplant

20m

Rome

Talks Oral contributions

Sprecher

Jussi Toppari (Nanoscience Center and Department of Physics, University of Jyväskylä, Finland)

Beschreibung

Recently, periodic nanostructures made of DNA origami tiles have become a viable option for applications in fields like diagnostics, electronics, sensing and optics, especially in challenging applications like the creating metasurfaces with unique optical properties.$^1$ Main benefit with origami is its superior self-assembly properties and vast variety of functionalization schemes,$^2$ and in particular the possibility to assembly them into larger nanostructured constructions with precision comparable to e-beam lithography while avoiding the slow and expensive patterning. Self-assembled DNA origami lattices with large surface coverage and high order, have been achieved mostly on very smooth substrates, like mica or lipid layers,$^3$ which usually are not compatible with any traditional nanofabrication process. To overcome this limitation and allow further processing, e.g., by DNA-assisted lithography,$^4$ we have demonstrated large-scale assembly of 2D fishnet-type lattices on a silicon substrate using cross-shaped DNA origami, so-called Seeman tile, as the building block.$^5$

Here, we further investigate how different parameters, like tile-tile and tile-surface interaction strengths, temperature, concentrations and time, affect the assembly, concentrating especially on how the size of the single crystalline domains within the formed polycrystalline type of layer can be increased towards completely single crystalline lattice. Results are analysed for precision of formation, surface coverage of well-formed lattice and achieved domain size. For this, systematic statistical counting as well as FFT analysis for periodicity and correlation lengths were made from AFM data. These origami-lattices on silicon substrate can be further used for any traditional fabrication processes like etching or metallization, to turn them for example into metamaterial with novel optical properties.

1. L. Lermusiaux et al. Nano Ex. 3:021003 (2022).
2. F. Hong et al. 117:12584 (2017).
3. J.M. Parikka et al. Molecules 26:1502 (2021).
4. B. Shen et al. Science Adv. 4:eaap8978 (2018).
5. K. Tapio et al. Chem. Mater. 35:1961 (2023).