Sprecher
Beschreibung
The ability to precisely control DNA origami orientation holds immense potential for a wide range of applications.1 This includes the development of advanced metamaterials, highly sensitive chiral sensing platforms, high-density data storage devices, and sophisticated drug delivery systems. Any method to achieve DNA origami orientation control is therefore attractive for both fundamental research and technological innovation.
This presentation explains the simple yet powerful approach to control the orientation of DNA origami nanostructures upon deposition on different substrates. By varying the Mg²⁺ concentration of the buffer solution, we demonstrate the ability to control the orientation of a chiral 2D DNA origami shape on the mica surface (Figure 1). A chiral double-L (CDL) DNA origami structure was used that can adopt either an S or Z orientation upon adsorption. CDL adsorption on mica was probed by atomic force microscopy (AFM), both for dried samples as well as at the liquid-solid interface. Distributions of S and Z orientations are shown to depend dramatically on the Mg2+ concentration, ranging from randomly oriented CDLs to exclusive S. The results are explained by considering Mg²⁺ induced conformational transitions in the 3D shape of the 2D CDL DNA origami.
In the second part of the presentation, the influence of different substrates such as silica, graphite, and graphene on S or Z orientation will be discussed.
